@article{fdi:010053444,
  title = {{D}ifferential expression of salivary proteins between susceptible and insecticide-resistant mosquitoes of {C}ulex quinquefasciatus},
  author = {{D}jegbe, {I}. and {C}orn{\'e}lie, {S}ylvie and {R}ossignol, {M}. and {D}emettre, {E}. and {S}eveno, {M}. and {R}emou{\'e}, {F}ranck and {C}orbel, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he {C}ulex quinquefasciatus mosquito, a major pest and vector of filariasis and arboviruses in the tropics, has developed multiple resistance mechanisms to the main insecticide classes currently available in public health. {A}mong them, the insensitive acetylcholinesterase (ace-1({R}) allele) is widespread worldwide and confers cross-resistance to organophosphates and carbamates. {F}ortunately, in an insecticide-free environment, this mutation is associated with a severe genetic cost that can affect various life history traits. {S}alivary proteins are directly involved in human-vector contact during biting and therefore play a key role in pathogen transmission. {M}ethods and {R}esults: {A}n original proteomic approach combining 2{D}-electrophoresis and mass spectrometry was adopted to compare the salivary expression profiles of two strains of {C}. quinquefasciatus with the same genetic background but carrying either the ace-1({R}) resistance allele or not (wild type). {F}our salivary proteins were differentially expressed (> 2 fold, {P} < 0.05) in susceptible ({SLAB}) and resistant ({SR}) mosquito strains. {P}rotein identification indicated that the {D}7 long form, a major salivary protein involved in blood feeding success, presented lower expression in the resistant strain than the susceptible strain. {I}n contrast, three other proteins, including metabolic enzymes (endoplasmin, triosephosphate isomerase) were significantly over-expressed in the salivary gland of ace-1({R}) resistant mosquitoes. {A} catalogue of 67 salivary proteins of {C}. quinquefasciatus sialotranscriptome was also identified and described. {C}onclusion: {T}he "resistance"-dependent expression of salivary proteins in mosquitoes may have considerable impact on biting behaviour and hence on the capacity to transmit parasites/viruses to humans. {T}he behaviour of susceptible and insecticide-resistant mosquitoes in the presence of vertebrate hosts and its impact on pathogen transmission urgently requires further investigation.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {6},
  numero = {3},
  pages = {e17496},
  ISSN = {1932-6203},
  year = {2011},
  DOI = {10.1371/journal.pone.0017496},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053444},
}