Verchot L. V. auteur aut IRD Dutaur L. auteur aut IRD Shepherd K. D. auteur aut IRD Albrecht Alain auteur aut IRD text journalArticle eng text/pdf born digital access We studied the biochemical and biophysical processes of carbon sequestration in an intensive agroforestry system on two soils (Feralsol - Luero; Arenosol - Teso) in W. Kenya to elucidate the mechanisms associated with long-term carbon storage. Specifically, we looked at a top-down model (macro-aggregates form around organic matter particles and micro-aggregates form within the macro-aggregates) and a bottom-up model (micro-aggregates form independently and are incorporated into macro-aggregates) of soil aggregate formation. Soil samples were collected from experiments on improved tree fallows using different species and two tillage treatments; water-stable aggregates were extracted and sorted into three size classes: macro-aggregates (>212 mu m), meso-aggregates (53-212 mu m) and micro-aggregates (20-53 mu m). Organic matter characterization of each fraction was based on C-13 isotope abundance, Fourier transform infrared (FTIR) spectroscopy and the abundance of polysaccharides. Improved fallows increased soil C by 0.28 and 0.26 kg m(-2) in the top 20 cm of the soil profile in Luero and Teso, respectively. Tillage altered the distribution of aggregates among size classes. Changes in the delta C-13 signature in each fraction indicated that more of the new carbon was found in the macro-aggregates (35-70%) and mesa-aggregates (18-49%) in Luero and less (9-17%) was found in the micro-aggregates. In Teso, about 40-80% of the new aggregate C was found in the mesa-aggregates. 14-45% was found in the micro-aggregates and only 4-26% was found in the macro-aggregates. The mesoaggregates and macro-aggregates to a lesser extent, in both sites, were enriched in carboxylic-C and aromatic-C, indicating the importance of OM decomposition and plant-derived C in the stabilization of larger aggregates, supporting the top-down model of aggregate formation. Microbially derived polysaccharides play a leading role in the formation of stable micro-aggregates and carboxylic-C promotes stabilization through surface occlusion. This bottom-up process is essential to promote long-term carbon sequestration in soils. Additionally, the micro-aggregates at both sites were enriched in polysaccharides and had elevated ratios of galactose + mannose:arabinose + xylose than the other aggregate fractions, indicating the importance of microbial processes in the formation of stable micro-aggregates and supporting the bottom-up model. specialized Carbon sequestration Carbohydrates C-13 FTIR Aggregates Improved fallow 068 161 3-4 182-193 2011 0016-7061 https://www.documentation.ird.fr/hor/fdi:010053417 10.1016/j.geoderma.2010.12.017 0016-7061 [F B010053417] https://www.documentation.ird.fr/hor/fdi:010053417 https://www.documentation.ird.fr/intranet/publi/2011/04/010053417.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-05-02 2017-08-23 fdi:010053417 fre