Dobigny Gauthier auteur aut IRD Poirier P. auteur aut IRD Hima Karmadine auteur aut IRD Cabaret O. auteur aut IRD Gauthier Philippe auteur aut IRD Tatard C. auteur aut IRD Costa J. M. auteur aut IRD Bretagne S. auteur aut IRD text journalArticle eng text/pdf born digital access Invading rodent species can harbor parasites with potential transmission to native rodents and/or humans. To investigate trypanosomes prevalence in rodents, the spleen of 76 rodents from Niger identified by their karyotype was used as a DNA source for Trypanosoma detection using a newly developed qPCR assay. Of the invasive black rat, Rattus rattus, 71% (10/14) were PCR positive as well as 6% (4/62) of native African rodents. Sequences of similar to 400 bp of the SSU rDNA gene identified phylogenetically close Trypanosoma lineages. Trypanosoma lewisi was present in all positive black rats and the sequences displayed 100% similarity with T. lewisi-infected humans in Senegal. T. lewisi was also detected in one Acomys johannis, suggesting a possible transmission to native species. In addition to improved knowledge of Trypanosoma diversity in rodents, our data underscore the introduction of the potentially pathogenic T. lewisi kinetoplastid through the human-mediated invasion of black rats all over West Africa. specialized Zoonotic emerging disease Biological invasion Rattus rattus Africa Quantitative real-time PCR 052 080 117 3 183-188 2011 0001-706X https://www.documentation.ird.fr/hor/fdi:010053379 10.1016/j.actatropica.2010.11.004 0001-706X [F B010053379] https://www.documentation.ird.fr/hor/fdi:010053379 https://www.documentation.ird.fr/intranet/publi/2011/03/010053379.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-04-07 2024-04-29 fdi:010053379 fre