Bouanane Darenfed Amel auteur aut IRD Fardeau Marie-Laure auteur aut IRD Grégoire Patrick auteur aut IRD Joseph Manon auteur aut IRD Kebbouche-Gana S. auteur aut IRD Benayad T. auteur aut IRD Hacene H. auteur aut IRD Cayol Jean-Luc auteur aut IRD Ollivier Bernard auteur aut IRD text journalArticle eng text/pdf born digital access A thermophilic anaerobic bacterium (strain TH7C1(T)) was isolated from the hydrothermal hot spring of Guelma in the northeast of Algeria. Strain TH7C1(T) stained Gram-positive, was a non-motile rod appearing singly, in pairs, or as long chains (0.7-1 x 2-6 mu m(2)). Spores were never observed. It grew at temperatures between 55 and 75A degrees C (optimum 65A degrees C) and at pH between 6.2 and 8.3 (optimum 6.9). It did not require NaCl for growth, but tolerated it up to 5 g l(-1). Strain TH7C1(T) is an obligatory heterotroph fermenting sugars including glucose, galactose, lactose, raffinose, fructose, ribose, xylose, arabinose, maltose, mannitol, cellobiose, mannose, melibiose, saccharose, but also xylan, and pyruvate. Fermentation of sugars only occurred in the presence of yeast extract (0.1%). The end-products from glucose fermentation were acetate, lactate, ethanol, CO2, and H-2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The G+C content of the genomic DNA was 44.7 mol% (HPLC techniques). Phylogenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence indicated that strain TH7C1(T) was affiliated to Firmicutes, order Clostridiales, family Caldicoprobacteraceae, with Caldicoprobacter oshimai (98.5%) being its closest relative. Based on phenotypic, phylogenetic, and genetic characteristics, strain TH7C1(T) is proposed as a novel species of genus Caldicoprobacter, Caldicoprobacter algeriensis, sp. nov. (strain TH7C1(T) = DSM 22661(T) = JCM 16184(T)). specialized 084 62 3 826-832 2011 0343-8651 https://www.documentation.ird.fr/hor/fdi:010053373 10.1007/s00284-010-9789-9 0343-8651 [F B010053373] https://www.documentation.ird.fr/hor/fdi:010053373 https://www.documentation.ird.fr/intranet/publi/2011/03/010053373.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-04-07 2017-08-23 fdi:010053373 fre