@article{fdi:010053346,
  title = {{B}acterial flora as indicated by {PCR}-temperature gradient gel electrophoresis ({TGGE}) of 16{S} r{DNA} gene fragments from isolated guts of phlebotomine sand flies ({D}iptera : {P}sychodidae)},
  author = {{G}uernaoui, {S}ouad and {G}arcia, {D}eborah and {G}azanion, {E}lodie and {O}uhdouch, {Y}. and {B}oumezzough, {A}. and {P}esson, {B}. and {F}ontenille, {D}idier and {S}ereno, {D}enis},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, we tested the capacity of {T}emperature {G}radient {G}el {E}lectrophoresis ({TGGE})-based fingerprinting of 16{S} r{DNA} {PCR} fragments to assess bacterial composition in a single isolated sand fly gut. {B}acterial content was studied in different life stages of a laboratory-reared colony of {P}hlebotomus duboscqi and in a wild-caught {P}hlebotomus papatasi population. {O}ur study demonstrates that a major reorganization in the gut bacterial community occurs during metamorphosis of sand flies. {C}hloroflexi spp. was dominant in the guts of pre-imaginal stages, although {M}icrobacterium spp. and another as yet unidentified bacteria were detected in the gut of the adult specimen. {I}nterestingly, {M}icrobacterium spp. was also found in all the adult guts of both species. {W}e demonstrate that the analysis of bacterial diversity in an individualized sand fly gut is possible with fingerprinting of 16{S} r{DNA}. {T}he use of such methodology, in conjunction with other culture-based methods, will be of great help in investigating the behavior of the {L}eishmania-bacterial community in an ecological context.},
  keywords = {{P}hlebotomine sand flies ; gut ; bacteria ; temperature gradient gel ; electrophoresis ({TGGE})},
  booktitle = {},
  journal = {{J}ournal of {V}ector {E}cology},
  volume = {36},
  numero = {{S}uppl. 1},
  pages = {{S}144--{S}147},
  ISSN = {1081-1710},
  year = {2011},
  DOI = {10.1111/j.1948-7134.2011.00124.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053346},
}