@article{fdi:010053345,
  title = {{P}atterns of sequence polymorphism in the fleshless berry locus in cultivated and wild {V}itis vinifera accessions},
  author = {{H}ouel, {C}. and {B}ounon, {R}. and {C}haib, {J}. and {G}uichard, {C}. and {P}eros, {J}. {P}. and {B}acilieri, {R}. and {D}ereeper, {A}lexis and {C}anaguier, {A}. and {L}acombe, {T}. and {N}'{D}iaye, {A}. and {L}e {P}aslier, {M}. {C}. and {V}ernerey, {M}. {S}. and {C}oriton, {O}. and {B}runel, {D}. and {T}his, {P}. and {T}orregrosa, {L}. and {A}dam-{B}london, {A}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {U}nlike in tomato, little is known about the genetic and molecular control of fleshy fruit development of perennial fruit trees like grapevine ({V}itis vinifera {L}.). {H}ere we present the study of the sequence polymorphism in a 1 {M}b grapevine genome region at the top of chromosome 18 carrying the fleshless berry mutation (flb) in order, first to identify {SNP} markers closely linked to the gene and second to search for possible signatures of domestication. {R}esults: {I}n total, 62 regions (17 {SSR}, 3 {SNP}, 1 {CAPS} and 41 re-sequenced gene fragments) were scanned for polymorphism along a 3.4 {M}b interval (85,127-3,506,060 bp) at the top of the chromosome 18, in both {V}. vinifera cv. {C}hardonnay and a genotype carrying the flb mutation, {V}. vinifera cv. {U}gni {B}lanc mutant. {A} nearly complete homozygosity in {U}gni {B}lanc (wild and mutant forms) and an expected high level of heterozygosity in {C}hardonnay were revealed. {E}xperiments using q{PCR} and {BAC} {FISH} confirmed the observed homozygosity. {U}nder the assumption that flb could be one of the genes involved into the domestication syndrome of grapevine, we sequenced 69 gene fragments, spread over the flb region, representing 48,874 bp in a highly diverse set of cultivated and wild {V}. vinifera genotypes, to identify possible signatures of domestication in the cultivated {V}. vinifera compartment. {W}e identified eight gene fragments presenting a significant deviation from neutrality of the {T}ajima's {D} parameter in the cultivated pool. {O}ne of these also showed higher nucleotide diversity in the wild compartments than in the cultivated compartments. {I}n addition, {SNP}s significantly associated to berry weight variation were identified in the flb region. {C}onclusions: {W}e observed the occurrence of a large homozygous region in a non-repetitive region of the grapevine otherwise highly-heterozygous genome and propose a hypothesis for its formation. {W}e demonstrated the feasibility to apply {BAC} {FISH} on the very small grapevine chromosomes and provided a specific probe for the identification of chromosome 18 on a cytogenetic map. {W}e evidenced genes showing putative signatures of selection and {SNP}s significantly associated with berry weight variation in the flb region. {I}n addition, we provided to the community 554 {SNP}s at the top of chromosome 18 for the development of a genotyping chip for future fine mapping of the flb gene in a {F}2 population when available.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {P}lant {B}iology},
  volume = {10},
  numero = {},
  pages = {284},
  ISSN = {1471-2229},
  year = {2010},
  DOI = {10.1186/1471-2229-10-284},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053345},
}