Haouari O. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Cayol Jean-Luc auteur aut IRD Casiot C. auteur aut IRD Elbaz-Poulichet F. auteur aut IRD Hamdi M. auteur aut IRD Joseph Manon auteur aut IRD Ollivier Bernard auteur aut IRD text journalArticle eng text/pdf born digital access A novel strictly anaerobic, moderately thermophilic, sulfate-reducing bacterium, designated strain Lam5(T), was isolated from a hot spring in north-east Tunisia and was characterized phenotypically and phylogenetically. The isolate stained Gram-negative but had a Gram-positive-type cell wall. The strain comprised endospore-forming, slightly curved rod-shaped cells with peritrichous flagella. It did not possess desulfoviridin. Strain Lam5(T) grew anaerobically at 40-60 degrees C (optimally at 55 degrees C) and at pH 5.8-8.2 (optimally at pH 7.1); it did not require NaCl but tolerated concentrations up to 1.5% (w/v). It utilized lactate, pyruvate, formate, ethanol, butanol, glycerol, propanol and H-2 (plus acetate) as electron donors. Lactate was oxidized and pyruvate was fermented to acetate. Sulfate, sulfite, thiosulfate, As(V) and Fe(III) (but not elemental sulfur, fumarate, nitrate or nitrite) were used as electron acceptors. The G+C content of the genomic DNA was 46.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain Lam5(T) was a member of the genus Desulfotomaculum, with Desulfotomaculum putei as its closest relative (96% similarity to the type strain). On the basis of genotypic, phenotypic and phylogenetic data, strain Lam5(T) represents a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum hydrothermale sp. nov. is proposed. The type strain is Lam5(T) (=DSM 18033(T) =JCM 13992(T)). specialized 084 020 58 Part 11 2529-2535 2008 1466-5026 https://www.documentation.ird.fr/hor/fdi:010053308 10.1099/ijs.0.65339-0 1466-5026 [F B010053308] https://www.documentation.ird.fr/hor/fdi:010053308 https://www.documentation.ird.fr/intranet/publi/depot/2011-11-18/010053308.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-02-06 2017-08-23 fdi:010053308 fre