@article{fdi:010053308, title = {{D}esulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial {T}unisian hot spring}, author = {{H}aouari, {O}. and {F}ardeau, {M}arie-{L}aure and {C}ayol, {J}ean-{L}uc and {C}asiot, {C}. and {E}lbaz-{P}oulichet, {F}. and {H}amdi, {M}. and {J}oseph, {M}anon and {O}llivier, {B}ernard}, editor = {}, language = {{ENG}}, abstract = {{A} novel strictly anaerobic, moderately thermophilic, sulfate-reducing bacterium, designated strain {L}am5({T}), was isolated from a hot spring in north-east {T}unisia and was characterized phenotypically and phylogenetically. {T}he isolate stained {G}ram-negative but had a {G}ram-positive-type cell wall. {T}he strain comprised endospore-forming, slightly curved rod-shaped cells with peritrichous flagella. {I}t did not possess desulfoviridin. {S}train {L}am5({T}) grew anaerobically at 40-60 degrees {C} (optimally at 55 degrees {C}) and at p{H} 5.8-8.2 (optimally at p{H} 7.1); it did not require {N}a{C}l but tolerated concentrations up to 1.5% (w/v). {I}t utilized lactate, pyruvate, formate, ethanol, butanol, glycerol, propanol and {H}-2 (plus acetate) as electron donors. {L}actate was oxidized and pyruvate was fermented to acetate. {S}ulfate, sulfite, thiosulfate, {A}s({V}) and {F}e({III}) (but not elemental sulfur, fumarate, nitrate or nitrite) were used as electron acceptors. {T}he {G}+{C} content of the genomic {DNA} was 46.8 mol%. {P}hylogenetic analysis based on 16{S} r{RNA} gene sequencing showed that strain {L}am5({T}) was a member of the genus {D}esulfotomaculum, with {D}esulfotomaculum putei as its closest relative (96% similarity to the type strain). {O}n the basis of genotypic, phenotypic and phylogenetic data, strain {L}am5({T}) represents a novel species of the genus {D}esulfotomaculum, for which the name {D}esulfotomaculum hydrothermale sp. nov. is proposed. {T}he type strain is {L}am5({T}) (={DSM} 18033({T}) ={JCM} 13992({T})).}, keywords = {}, booktitle = {}, journal = {{I}nternational {J}ournal of {S}ystematic and {E}volutionary {M}icrobiology}, volume = {58}, numero = {{P}art 11}, pages = {2529--2535}, ISSN = {1466-5026}, year = {2008}, DOI = {10.1099/ijs.0.65339-0}, URL = {https://www.documentation.ird.fr/hor/fdi:010053308}, }