Beulé Thierry auteur aut IRD Camps Céline auteur aut IRD Debiesse Ségolène auteur aut IRD Tranchant Christine auteur aut IRD Dussert Stéphane auteur aut IRD Sabau X. auteur aut IRD Jaligot Estelle auteur aut IRD Alwee Ssrs auteur aut IRD Tregear James auteur aut IRD text journalArticle eng text/pdf born digital access Micropropagation of oil palm by somatic embryogenesis produces a proportion of off-type individuals (approximately 5% overall) displaying a homeotically modified flower structure known as mantled. Transformation of the fertile or sterile androecium into carpel-like structures is observed in staminate and pistillate mantled flowers, respectively, resulting in lower oil yields in affected plantations. Given the epigenetic nature of the mantled condition, a gene expression-based approach was used rather than a genetic one to investigate its molecular basis. Suppression subtractive hybridisation (SSH) and macroarray hybridisation were used to compare transcriptome patterns between normal and mantled inflorescences. Two SSH libraries, enriched for complementary deoxyribonucleic acids (cDNAs) of either true-to-type or somaclonal variant material, were generated. Bioinformatic analysis of these two libraries allowed the identification of 1,350 unique sequences and their annotation by a gene ontology-based approach. Macroarray hybridisation was used to compare gene expression between normal and mantled inflorescences, and 32 genes were found to be differentially expressed. The temporal expression patterns of six genes were further investigated in more detail in relation to male and female inflorescence development. Full-length cDNAs were isolated and characterised for two of these genes, EgFB1 and EgRING1, both of which are down-regulated in the mantled inflorescences and both of which encode proteins associated with proteolytic signalling complexes. Our data shed light on gene expression changes associated with the mantled phenotype and have provided novel transcriptome markers which can help to distinguish the abnormal and wild-type inflorescences. specialized Elaeis guineensis Mantled Expressed sequence tag F-box protein RING finger protein Somaclonal variation 076 7 1 169-182 2011 1614-2942 https://www.documentation.ird.fr/hor/fdi:010053162 10.1007/s11295-010-0323-9 1614-2942 [F B010053162] https://www.documentation.ird.fr/hor/fdi:010053162 https://www.documentation.ird.fr/intranet/publi/2011/03/010053162.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-04-07 2017-08-23 fdi:010053162 fre