@article{fdi:010053162,
  title = {{T}ranscriptome analysis reveals differentially expressed genes associated with the mantled homeotic flowering abnormality in oil palm ({E}laeis guineensis)},
  author = {{B}eul{\'e}, {T}hierry and {C}amps, {C}{\'e}line and {D}ebiesse, {S}{\'e}gol{\`e}ne and {T}ranchant, {C}hristine and {D}ussert, {S}t{\'e}phane and {S}abau, {X}. and {J}aligot, {E}stelle and {A}lwee, {S}srs and {T}regear, {J}ames},
  editor = {},
  language = {{ENG}},
  abstract = {{M}icropropagation of oil palm by somatic embryogenesis produces a proportion of off-type individuals (approximately 5% overall) displaying a homeotically modified flower structure known as mantled. {T}ransformation of the fertile or sterile androecium into carpel-like structures is observed in staminate and pistillate mantled flowers, respectively, resulting in lower oil yields in affected plantations. {G}iven the epigenetic nature of the mantled condition, a gene expression-based approach was used rather than a genetic one to investigate its molecular basis. {S}uppression subtractive hybridisation ({SSH}) and macroarray hybridisation were used to compare transcriptome patterns between normal and mantled inflorescences. {T}wo {SSH} libraries, enriched for complementary deoxyribonucleic acids (c{DNA}s) of either true-to-type or somaclonal variant material, were generated. {B}ioinformatic analysis of these two libraries allowed the identification of 1,350 unique sequences and their annotation by a gene ontology-based approach. {M}acroarray hybridisation was used to compare gene expression between normal and mantled inflorescences, and 32 genes were found to be differentially expressed. {T}he temporal expression patterns of six genes were further investigated in more detail in relation to male and female inflorescence development. {F}ull-length c{DNA}s were isolated and characterised for two of these genes, {E}g{FB}1 and {E}g{RING}1, both of which are down-regulated in the mantled inflorescences and both of which encode proteins associated with proteolytic signalling complexes. {O}ur data shed light on gene expression changes associated with the mantled phenotype and have provided novel transcriptome markers which can help to distinguish the abnormal and wild-type inflorescences.},
  keywords = {{E}laeis guineensis ; {M}antled ; {E}xpressed sequence tag ; {F}-box protein ; {RING} ; finger protein ; {S}omaclonal variation},
  booktitle = {},
  journal = {{T}ree {G}enetics and {G}enomes},
  volume = {7},
  numero = {1},
  pages = {169--182},
  ISSN = {1614-2942},
  year = {2011},
  DOI = {10.1007/s11295-010-0323-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053162},
}