@article{fdi:010053146,
  title = {{A} multiplex assay for the simultaneous detection of antibodies against 15 {P}lasmodium falciparum and {A}nopheles gambiae saliva antigens},
  author = {{A}mbrosino, {E}. and {D}umoulin, {C}. and {O}rlandi-{P}radines, {E}. and {R}emou{\'e}, {F}ranck and {T}oure-{B}alde, {A}. and {T}all, {A}. and {S}arr, {J}ean {B}iram and {P}oinsignon, {A}nne and {S}okhna, {C}heikh and {P}uget, {K}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {P}ascual, {A}. and {D}ruilhe, {P}. and {F}usai, {T}. and {R}ogier, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {A}ssessment exposure and immunity to malaria is an important step in the fight against the disease. {I}ncreased malaria infection in non-immune travellers under anti-malarial chemoprophylaxis, as well as the implementation of malaria elimination programmes in endemic countries, raises new issues that pertain to these processes. {N}otably, monitoring malaria immunity has become more difficult in individuals showing low antibody ({A}b) responses or taking medications against the {P}lasmodium falciparum blood stages. {C}ommonly available techniques in malaria seroepidemiology have limited sensitivity, both against pre-erythrocytic, as against blood stages of the parasite. {T}hus, the aim of this study was to develop a sensitive tool to assess the exposure to malaria or to bites from the vector {A}nopheles gambiae, despite anti-malarial prophylactic treatment. {M}ethods: {A}b responses to 13 pre-erythrocytic {P}. falciparum-specific peptides derived from the proteins {L}sa1, {L}sa3, {G}lurp, {S}alsa, {T}rap, {S}tarp, {CSP} and {P}f11.1, and to 2 peptides specific for the {A}nopheles gambiae saliva protein g{SG}6 were tested. {I}n this study, 253 individuals from three {S}enegalese areas with different transmission intensities and 124 {E}uropean travellers exposed to malaria during a short period of time were included. {R}esults: {T}he multiplex assay was optimized for most but not all of the antigens. {I}t was rapid, reproducible and required a small volume of serum. {P}roportions of {A}b-positive individuals, {A}b levels and the mean number of antigens ({A}gs) recognized by each individual increased significantly with increases in the level of malaria exposure. {C}onclusion: {T}he multiplex assay developed here provides a useful tool to evaluate immune responses to multiple {A}gs in large populations, even when only small amounts of serum are available, or {A}b titres are low, as in case of travellers. {F}inally, the relationship of {A}b responses with malaria endemicity levels provides a way to monitor exposure in differentially exposed autochthonous individuals from various endemicity areas, as well as in travellers who are not immune, thus indirectly assessing the parasite transmission and malaria risk in the new eradication era.},
  keywords = {},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {9},
  numero = {},
  pages = {317},
  ISSN = {1475-2875},
  year = {2010},
  DOI = {10.1186/1475-2875-9-317},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053146},
}