Rezgui R. auteur aut IRD Gam Z.B. auteur aut IRD Ben Hamed S. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Cayol J.L. auteur aut IRD Maaroufi A. auteur aut IRD Labat Marc auteur aut IRD text journalArticle eng text/pdf born digital access A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37(T), was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37(T) stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37(T) had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C-15:0 (41 %), iso-C-14:0 3-OH and/or iso-C-15:0 dimethyl acetal (21.6%), iso-C-13:0 (4.4%), anteiso-C-15:0 (3.9%) and iso-C-15:1 (2.8%). Strain SOL3f37(T) grew between 20 and 48 degrees C (optimum 40 degrees C) and at pH 6.2-8.1 (optimum pH 6.9). Strain SOL3f37(T) required at least 0.5 g NaCl l(-1) and grew in the presence of NaCI concentrations up to 150 g (optimum 40 g l(-1)). Yeast extract (2 g l(-1)) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37(T) grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37(T) was a member of the family Clostridiaceae in the order Clostridiales; strain SOL3f37(T) was related to members of various genera of the family Clostridiaceae. It exhibited highest 16S rRNA gene sequence similarity (93.4%) with Clostridiisalibacter paucivorans 37HS60(T), 91.8% with Thermohalobacter berrensis CTT3(T) and 91.7% with Caloranaerobacter azorensis MV1087(T). On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37(T) represents a novel species in a new genus. The name Sporosalibacterium faouarense gen. nov., sp. nov. is proposed, with SOL3f37(T) (=DSM 21485(T) =JCM 15487(T)) as the type strain of Sporosalibacterium faouarense. specialized TUNISIE 084 61 Part 1 99-104 2011 1466-5026 https://www.documentation.ird.fr/hor/fdi:010053141 10.1099/ijs.0.017715-0 1466-5026 [F B010053141] https://www.documentation.ird.fr/hor/fdi:010053141 https://www.documentation.ird.fr/intranet/publi/2011/03/010053141.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-04-07 2017-08-23 fdi:010053141 fre