Carrier G. auteur aut IRD Santoni S. auteur aut IRD Rodier-Goud M. auteur aut IRD Canaguier A. auteur aut IRD Kochko Alexandre de auteur aut IRD Tranchant Dubreuil Christine auteur aut IRD This P. auteur aut IRD Boursiquot J.M. auteur aut IRD Le Cunff L. auteur aut IRD text journalArticle eng text/pdf born digital access Premise of the study : In this study, we developed a nuclear DNA extraction protocol for Next Generation Sequencers (NGS). Methods and Results : We applied this extraction method to grapevines and coffee trees, which are known to contain many secondary metabolites. The nuclear DNA obtained was sequenced by the 454/GS-FLX method. We obtained excellent results, with less than 4% cytoplasmic DNA, in a similar way to a BAC (Bacterial Artificial Chromosome)-building protocol. We also compared our protocol with a classic DNA extraction using specific cytoplasmic DNA amplification. Results showed a lower cytoplasmic DNA contamination with the new protocol. Conclusions : The method presented here is fast and economical. The DNA obtained is of high quality, with a low level of cytoplasmic DNA contamination, and very efficient for the construction of sequencing libraries. specialized next generation sequencers nuclear plant DNA extraction nuclei isolation 076 98 1 E13-E15 2011 0002-9122 https://www.documentation.ird.fr/hor/fdi:010053128 10.3732/ajb.1000371 0002-9122 [F B010053128] https://www.documentation.ird.fr/hor/fdi:010053128 https://www.documentation.ird.fr/intranet/publi/2011/03/010053128.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-04-07 2017-08-23 fdi:010053128 fre