Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES American Journal of Botany E13-E15 next generation sequencers nuclear plant DNA extraction nuclei isolation 2011 fdi:010053128 ENG American Journal of Botany 0002-9122 ISI:000285747900005 1 10.3732/ajb.1000371 https://www.documentation.ird.fr/hor/fdi:010053128 https://www.documentation.ird.fr/intranet/publi/2011/03/010053128.pdf 98 Horizon (IRD) Premise of the study : In this study, we developed a nuclear DNA extraction protocol for Next Generation Sequencers (NGS). Methods and Results : We applied this extraction method to grapevines and coffee trees, which are known to contain many secondary metabolites. The nuclear DNA obtained was sequenced by the 454/GS-FLX method. We obtained excellent results, with less than 4% cytoplasmic DNA, in a similar way to a BAC (Bacterial Artificial Chromosome)-building protocol. We also compared our protocol with a classic DNA extraction using specific cytoplasmic DNA amplification. Results showed a lower cytoplasmic DNA contamination with the new protocol. Conclusions : The method presented here is fast and economical. The DNA obtained is of high quality, with a low level of cytoplasmic DNA contamination, and very efficient for the construction of sequencing libraries. 076 UR188