%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Carrier, G.
%A Santoni, S.
%A Rodier-Goud, M.
%A Canaguier, A.
%A Kochko, Alexandre de
%A Tranchant Dubreuil, Christine
%A This, P.
%A Boursiquot, J.M.
%A Le Cunff, L.
%T An efficient and rapid protocol for plant nuclear DNA preparation suitable for next generation sequencing methods
%D 2011
%L fdi:010053128
%G ENG
%J American Journal of Botany
%@ 0002-9122
%K next generation sequencers ; nuclear plant DNA extraction ; nuclei ; isolation
%M ISI:000285747900005
%N 1
%P E13-E15
%R 10.3732/ajb.1000371
%U https://www.documentation.ird.fr/hor/fdi:010053128
%> https://www.documentation.ird.fr/intranet/publi/2011/03/010053128.pdf
%V 98
%W Horizon (IRD)
%X Premise of the study : In this study, we developed a nuclear DNA extraction protocol for Next Generation Sequencers (NGS). Methods and Results : We applied this extraction method to grapevines and coffee trees, which are known to contain many secondary metabolites. The nuclear DNA obtained was sequenced by the 454/GS-FLX method. We obtained excellent results, with less than 4% cytoplasmic DNA, in a similar way to a BAC (Bacterial Artificial Chromosome)-building protocol. We also compared our protocol with a classic DNA extraction using specific cytoplasmic DNA amplification. Results showed a lower cytoplasmic DNA contamination with the new protocol. Conclusions : The method presented here is fast and economical. The DNA obtained is of high quality, with a low level of cytoplasmic DNA contamination, and very efficient for the construction of sequencing libraries.
%$ 076