@article{fdi:010053128,
  title = {{A}n efficient and rapid protocol for plant nuclear {DNA} preparation suitable for next generation sequencing methods},
  author = {{C}arrier, {G}. and {S}antoni, {S}. and {R}odier-{G}oud, {M}. and {C}anaguier, {A}. and {K}ochko, {A}lexandre de and {T}ranchant {D}ubreuil, {C}hristine and {T}his, {P}. and {B}oursiquot, {J}.{M}. and {L}e {C}unff, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}remise of the study : {I}n this study, we developed a nuclear {DNA} extraction protocol for {N}ext {G}eneration {S}equencers ({NGS}). {M}ethods and {R}esults : {W}e applied this extraction method to grapevines and coffee trees, which are known to contain many secondary metabolites. {T}he nuclear {DNA} obtained was sequenced by the 454/{GS}-{FLX} method. {W}e obtained excellent results, with less than 4% cytoplasmic {DNA}, in a similar way to a {BAC} ({B}acterial {A}rtificial {C}hromosome)-building protocol. {W}e also compared our protocol with a classic {DNA} extraction using specific cytoplasmic {DNA} amplification. {R}esults showed a lower cytoplasmic {DNA} contamination with the new protocol. {C}onclusions : {T}he method presented here is fast and economical. {T}he {DNA} obtained is of high quality, with a low level of cytoplasmic {DNA} contamination, and very efficient for the construction of sequencing libraries.},
  keywords = {next generation sequencers ; nuclear plant {DNA} extraction ; nuclei ; isolation},
  booktitle = {},
  journal = {{A}merican {J}ournal of {B}otany},
  volume = {98},
  numero = {1},
  pages = {{E}13--{E}15},
  ISSN = {0002-9122},
  year = {2011},
  DOI = {10.3732/ajb.1000371},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053128},
}