@article{fdi:010053120,
  title = {{N}ew cultural approaches for microaerophilic hyperthermophiles},
  author = {{U}zarraga, {R}. and {A}uria, {R}ichard and {D}avidson, {S}. and {N}avarro, {D}. and {C}ombet-{B}lanc, {Y}annick},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his article reports on a new culture system designed for studying the effects of nutritional factors on the growth of hyperthermophilic and chemolithotrophic microorganisms. {T}he system comprises 5-l stainless steel jars, an automatic gas dispenser, propylene microplates, and a robotic platform. {T}he culture system was validated using {A}quifex aeolicus, a hyperthermophilic, chemolithotrophic, and microaerophilic bacterium, which requires hydrogen, oxygen, {CO}2, and minerals for growth. {W}e demonstrated that the cell densities measured on 147 cultures of {A}. aeolicus microplated in jar at 80{A} degrees {C} under partial pressures (in k{P}a) of water vapor (47), {H}-2 (117.7), {O}-2 (28.1), {CO}2 (31.4), and {N}-2 (3.9), followed a normal distribution, with a mean of 0.72 and a standard deviation of 0.04 (variation coefficient: 5.7%). {I}n addition, cross-comparison of the growth kinetics of {A}. aeolicus in serum bottles and in a jar system highlighted similar kinetics patterns (both mean growth rates were 0.18 and 0.17 h-1, respectively), whereas the maximum cell densities reached were slightly lower in jar than in bottle (0.73 vs. 0.88 {OD} units, respectively). {F}urthermore, these results showed that, contrary to bottles, the total pressure of gas in jars remained constant throughout the biotic experiments, even with seven microplates completely filled with grown cultures. {I}n addition, this system has been validated also for hyperthermophilic strictly anaerobes such as {T}hermotoga maritima or aerobes such as {S}ulfolobus solfataricus. {T}his new culture system offers an interesting alternative for cultivating hyperthermophiles, using gas as substrate under constant pressure, thus making it possible to miniaturize experiments and study a large number of nutritional factors in one experimental run.},
  keywords = {},
  booktitle = {},
  journal = {{C}urrent {M}icrobiology},
  volume = {62},
  numero = {2},
  pages = {346--350},
  ISSN = {0343-8651},
  year = {2011},
  DOI = {10.1007/s00284-010-9712-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053120},
}