@article{fdi:010053113,
  title = {{C}ryopreservation of hairy roots of {R}ubia akane ({N}akai) using a droplet-vitrification procedure},
  author = {{K}im, {H}. {H}. and {P}opova, {E}. {V}. and {Y}i, {J}. {Y}. and {C}ho, {G}. {T}. and {P}ark, {S}. {U}. and {L}ee, {S}. {C}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A}n efficient protocol for the cryopreservation of madder ({R}ubia akane {N}akai) hairy root cultures was developed using droplet-vitrification and alternative loading and vitrification solutions formulated previously in our laboratory. {A}mong eight preculture treatments tested, the highest post-cryopreservation regeneration was obtained for explants incubated in liquid half-strength {MS} medium with progressively increased sucrose concentration (0.3 {M} for 54 h, then 0.5 {M} for 16 h). {L}oading of precultured explants improved their post-cryopreservation regeneration by 50-75% compared with non-loaded control. {C}ombining loading solution {C}4 (35% {PVS}3) and vitrification solution {B}5 (80% {PVS}3) was the most effective, while applying six {PVS}2-based solutions at room temperature resulted in low post-cryo regeneration. {T}reatment duration was optimized to 30 min for loading and to 10-20 min for vitrification solution. {A}pices of primary and secondary hairy roots showed similar post-cryo regeneration (88 and 95%, respectively), which was significantly higher than regeneration of root sections without apices (65%). {D}roplet-vitrification produced higher post-cryo regeneration than 'classical' vitrification in cryovials. {O}ur results suggest that droplet-vitrification using alternative loading and vitrification solutions is an efficient method for cryopreservation of {R}. akane hairy root cultures.},
  keywords = {droplet-vitrification ; hairy roots ; loading ; preculture ; {R}ubia akane ; vitrification solution},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {31},
  numero = {6},
  pages = {473--484},
  ISSN = {0143-2044},
  year = {2010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053113},
}