@article{fdi:010053096,
  title = {{I}nduction of a peptide with activity against a broad spectrum of pathogens in the {A}edes aegypti salivary gland, following {I}nfection with dengue virus},
  author = {{L}uplertlop, {N}. and {S}urasombatpattana, {P}ornapat and {P}atramool, {S}irilaksana and {D}umas, {E}milie and {W}asinpiyamongkol, {L}. and {S}aune, {L}aure and {H}amel, {R}odolphe and {B}ernard, {E}. and {S}ereno, {D}enis and {T}homas, {F}. and {P}iquemal, {D}. and {Y}ssel, {H}. and {B}riant, {L}. and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ultimate stage of the transmission of {D}engue {V}irus ({DENV}) to man is strongly dependent on crosstalk between the virus and the immune system of its vector {A}edes aegypti ({A}e. aegypti). {I}nfection of the mosquito's salivary glands by {DENV} is the final step prior to viral transmission. {T}herefore, in the present study, we have determined the modulatory effects of {DENV} infection on the immune response in this organ by carrying out a functional genomic analysis of uninfected salivary glands and salivary glands of female {A}e. aegypti mosquitoes infected with {DENV}. {W}e have shown that {DENV} infection of salivary glands strongly up-regulates the expression of genes that encode proteins involved in the vector's innate immune response, including the immune deficiency ({IMD}) and {T}oll signalling pathways, and that it induces the expression of the gene encoding a putative anti-bacterial, cecropin-like, peptide ({AAEL}000598). {B}oth the chemically synthesized non-cleaved, signal peptide-containing gene product of {AAEL}000598, and the cleaved, mature form, were found to exert, in addition to antibacterial activity, anti-{DENV} and anti-{C}hikungunya viral activity. {H}owever, in contrast to the mature form, the immature cecropin peptide was far more effective against {C}hikungunya virus ({CHIKV}) and, furthermore, had strong anti-parasite activity as shown by its ability to kill {L}eishmania spp. {R}esults from circular dichroism analysis showed that the immature form more readily adopts a helical conformation which would help it to cause membrane permeabilization, thus permitting its transfer across hydrophobic cell surfaces, which may explain the difference in the anti-pathogenic activity between the two forms. {T}he present study underscores not only the importance of {DENV}-induced cecropin in the innate immune response of {A}e. aegypti, but also emphasizes the broad-spectrum anti-pathogenic activity of the immature, signal peptide-containing form of this peptide.},
  keywords = {{ASIE}},
  booktitle = {},
  journal = {{P}los {P}athogens},
  volume = {7},
  numero = {1},
  pages = {art. e1001252},
  ISSN = {1553-7366},
  year = {2011},
  DOI = {10.1371/journal.ppat.1001252},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053096},
}