%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ozudogru, E. A.
%A Kirdok, E.
%A Kaya, E.
%A Capuana, M.
%A De Carlo, A.
%A Engelmann, Florent
%T Medium-term conservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro shoot cultures and encapsulated buds
%D 2011
%L fdi:010053063
%G ENG
%J Scientia Horticulturae
%@ 0304-4238
%K Cold storage ; Genetic resources ; In vitro shoot cultures ; Synthetic seeds
%M ISI:000286712300037
%N 3
%P 431-435
%R 10.1016/j.scienta.2010.10.013
%U https://www.documentation.ird.fr/hor/fdi:010053063
%> https://www.documentation.ird.fr/intranet/publi/2011/03/010053063.pdf
%V 127
%W Horizon (IRD)
%X This study evaluated the survival and recovery of non-encapsulated and encapsulated shoots of Sequoia sempervirens after storage at 4 degrees C in the dark for up to 15 months on four different culture media. Survival and regrowth of encapsulated shoots declined within 3 months, regardless of the storage medium composition. By contrast, no significant decrease in survival and regrowth was noted with non-encapsulated shoots after 12 months of storage on Quoirin and Lepoivre medium supplemented, or not, with 1 mg l(-1) benzyladenine. Regrowth dropped to 60-61% after 15 months of storage on the same media. Medium-term conservation of S. sempervirens germplasm is therefore possible using in vitro storage of non-encapsulated shoot cultures.
%$ 076