Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach Demaneche S. auteur aut IRD Philippot L. auteur aut IRD David M. M. auteur aut IRD Navarro Elisabeth auteur aut IRD Vogel T. M. auteur aut IRD Simonet P. auteur aut IRD text journalArticle eng text/pdf born digital access We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters. specialized 074 084 Applied and Environmental Microbiology 75 2 534-537 2009 0099-2240 https://www.documentation.ird.fr/hor/fdi:010053047 10.1128/aem.01706-08 0099-2240 [F B010053047] https://www.documentation.ird.fr/hor/fdi:010053047 https://www.documentation.ird.fr/intranet/publi/2009/compl3/010053047.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2011-03-23 2017-08-23 fdi:010053047 fre