%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Demaneche, S. %A Philippot, L. %A David, M. M. %A Navarro, Elisabeth %A Vogel, T. M. %A Simonet, P. %T Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach %D 2009 %L fdi:010053047 %G ENG %J Applied and Environmental Microbiology %@ 0099-2240 %M ISI:000262197700031 %N 2 %P 534-537 %R 10.1128/aem.01706-08 %U https://www.documentation.ird.fr/hor/fdi:010053047 %> https://www.documentation.ird.fr/intranet/publi/2009/compl3/010053047.pdf %V 75 %W Horizon (IRD) %X We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters. %$ 074 ; 084