%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Becquart, Pierre
%A Wauquier, N.
%A Nkoghe, D.
%A Ndjoyi-Mbiguino, A.
%A Padilla, C.
%A Souris, Marc
%A Leroy, Eric
%T Acute dengue virus 2 infection in Gabonese patients is associated with an early innate immune response, including strong interferon alpha production
%D 2010
%L fdi:010053024
%G ENG
%J Bmc Infectious Diseases
%@ 1471-2334
%M ISI:000285846000001
%P 356
%R 10.1186/1471-2334-10-356
%U https://www.documentation.ird.fr/hor/fdi:010053024
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-09/010053024.pdf
%V 10
%W Horizon (IRD)
%X Background: Dengue is now a leading cause of morbidity and mortality throughout the tropics. We conducted the first ex vivo study of dengue fever (DF) in African patients infected during the first Gabonese dengue virus 2 (DENV-2) outbreak in 2007, in order to investigate cytokine production, including the antiviral cytokine IFN-alpha, reported to be a potent inhibitor of DENV replication in vitro. Methods: Levels of 50 cytokines, chemokines and growth factors were measured in plasma from 36 patients with DENV-2 infection, and in uninfected controls, using Luminex multiplex technology. The results were interpreted according to the day of sampling after symptom onset. PBMC from six patients were also studied for T lymphocyte cell surface marker expression by flow cytometry. Results: Acute DENV-2 infection elicited high levels of several pro-inflammatory cytokines (IL-6 and IL-17), chemokines (MIF, RANTES, IP-10 and MCP-1) and growth factors (G-CSF, GM-CSF and VEGF-A). We also observed high levels of IFN-a for the first time in adult DF patients, and CD4+ and CD8+ T cell activation at symptom onset. Conclusion: Acute DENV-2 infection in African patients elicits a strong innate response involving IFN-a production, as well as an adaptive immune response.
%$ 052