@article{fdi:010053024,
  title = {{A}cute dengue virus 2 infection in {G}abonese patients is associated with an early innate immune response, including strong interferon alpha production},
  author = {{B}ecquart, {P}ierre and {W}auquier, {N}. and {N}koghe, {D}. and {N}djoyi-{M}biguino, {A}. and {P}adilla, {C}. and {S}ouris, {M}arc and {L}eroy, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}engue is now a leading cause of morbidity and mortality throughout the tropics. {W}e conducted the first ex vivo study of dengue fever ({DF}) in {A}frican patients infected during the first {G}abonese dengue virus 2 ({DENV}-2) outbreak in 2007, in order to investigate cytokine production, including the antiviral cytokine {IFN}-alpha, reported to be a potent inhibitor of {DENV} replication in vitro. {M}ethods: {L}evels of 50 cytokines, chemokines and growth factors were measured in plasma from 36 patients with {DENV}-2 infection, and in uninfected controls, using {L}uminex multiplex technology. {T}he results were interpreted according to the day of sampling after symptom onset. {PBMC} from six patients were also studied for {T} lymphocyte cell surface marker expression by flow cytometry. {R}esults: {A}cute {DENV}-2 infection elicited high levels of several pro-inflammatory cytokines ({IL}-6 and {IL}-17), chemokines ({MIF}, {RANTES}, {IP}-10 and {MCP}-1) and growth factors ({G}-{CSF}, {GM}-{CSF} and {VEGF}-{A}). {W}e also observed high levels of {IFN}-a for the first time in adult {DF} patients, and {CD}4+ and {CD}8+ {T} cell activation at symptom onset. {C}onclusion: {A}cute {DENV}-2 infection in {A}frican patients elicits a strong innate response involving {IFN}-a production, as well as an adaptive immune response.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {I}nfectious {D}iseases},
  volume = {10},
  numero = {},
  pages = {356},
  ISSN = {1471-2334},
  year = {2010},
  DOI = {10.1186/1471-2334-10-356},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053024},
}