@article{fdi:010052979,
  title = {{D}ehydration improves cryopreservation of coconut ({C}ocos nucifera {L}.)},
  author = {{S}isunandar, and {S}opade, {P}. {A}. and {S}amosir, {Y}. {M}. {S}. and {R}ival, {A}lain and {A}dkins, {S}. {W}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ryopreservation of coconut can be used as a strategy to back up the establishment of living collections which are expensive to maintain and are under constant threat from biotic and abiotic factors. {U}nfortunately, cryopreservation protocols still need to be developed that are capable of producing a sizeable number of field-grown plants. {T}herefore, we report on the development of an improved cryopreservation protocol which can be used on a wide range of coconut cultivars. {T}he cryopreservation of zygotic embryos and their recovery to soil-growing plants was achieved through the application of four optimised steps viz.: (i) rapid dehydration; (ii) rapid cooling; (iii) rapid warming and recovery in vitro and (iv) acclimatisation and soil-supported growth. {T}he thermal properties of water within the embryos were monitored using differential scanning calorimetry ({DSC}) in order to ensure that the freezable component was kept to a minimum. {T}he feasibility of the protocol was assessed using the {M}alayan {Y}ellow {D}warf ({MYD}) cultivar in {A}ustralia and then tested on a range of cultivars which were freshly harvested and studied in {I}ndonesia. {T}he most efficient protocol was one based on an 8-h rapid dehydration step followed by rapid cooling step. {B}est recovery percentages were obtained when a rapid warming step and an optimised in vitro culture step were used. {F}ollowing this protocol, 20% (when cryopreserved 12 days after harvesting) and 40% (when cryopreserved at the time of harvest) of all {MYD} embryos cryopreserved could be returned to normal seedlings growing in soil. {DSC} showed that this protocol induced a drop in embryo fresh weight to 19% and significantly reduced the amount of water remaining that could produce ice crystals (0.1%). {O}f the 20 cultivars tested, 16 were found to produce between 10% and 40% normal seedlings while four cultivars generated between 0% and 10% normal seedlings after cryopreservation. {T}his new protocol is applicable to a wide range of coconut cultivars and is useful for the routine cryopreservation of coconut genetic resources.},
  keywords = {{D}ehydration ; {D}ifferential scanning calorimetry ; {G}ermplasm conservation ; {E}mbryo culture ; {R}ecalcitrant seeds},
  booktitle = {},
  journal = {{C}ryobiology},
  volume = {61},
  numero = {3},
  pages = {289--296},
  ISSN = {0011-2240},
  year = {2010},
  DOI = {10.1016/j.cryobiol.2010.09.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052979},
}