@article{fdi:010052976,
  title = {{A} high-throughput method for early screening of coffee ({C}offea spp.) genotypes for resistance to root-knot nematodes ({M}eloidogyne spp.)},
  author = {{V}illain, {L}. and {A}ribi, {J}amel and {R}eversat, {G}eorges and {A}nthony, {F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{R}oot-knot nematodes ({M}eloidogyne spp.) threaten the livelihood of millions of farmers producing coffee worldwide. {T}he use of resistant plants either as cultivars or rootstocks appears to be the single most effective method of control. {A} screening method was developed to evaluate large populations of plants for resistance to root-knot nematodes. {T}wo coffee cultivars, one susceptible and the other resistant to {M}eloidogyne paranaensis, were grown under controlled conditions in two substrates: a commercial sieved potting compost and an inert substrate containing sand with a water-absorbent synthetic polymer. {P}lant growth and development and nematode multiplication were compared for two inoculation dates (2 and 8 weeks after planting) and two evaluation dates (eight and 13 weeks after inoculation). {R}oot growth, but not nematode multiplication, was influenced by the choice of substrate. {E}valuation of the differences in root weight and nematode numbers between the different cultivars, substrates and dates of inoculation suggested that an optimal condition could be defined. {T}he best discrimination between susceptible and resistant plants was found in the experiment where inoculation occurred at 2 weeks after planting and evaluation occurred at 8 weeks after inoculation. {B}ecause the total duration of this experiment was only 3 months, high-throughput evaluation was possible, opening up new possibilities for screening large germplasm collections and studying the genetic control of root-knot nematode resistance in coffee.},
  keywords = {{C}offee ; {M}eloidogyne ; {R}esistance assessment ; {R}oot-knot nematode ; {S}creening method},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {P}lant {P}athology},
  volume = {128},
  numero = {4},
  pages = {451--458},
  ISSN = {0929-1873},
  year = {2010},
  DOI = {10.1007/s10658-010-9671-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052976},
}