Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Journal of Allergy and Clinical Immunology 1294-U352 HIV children lymphocyte monocyte phenotyping long-term nonprogressors antiretroviral therapy Asia disease progression pediatric AIDS 2010 fdi:010052972 ENG Journal of Allergy and Clinical Immunology 0091-6749 ISI:000284947800030 6 10.1016/j.jaci.2010.09.038 https://www.documentation.ird.fr/hor/fdi:010052972 https://www.documentation.ird.fr/intranet/publi/2011/01/010052972.pdf 126 Horizon (IRD) Background: There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children. Objectives: To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls. Methods: We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4(+) T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children >= 8 years old with CD4(+) T-cell counts >= 350 cells/mm(3), were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53). Results: Compared with controls, HIV-positive children had lower values (cell count per mm(3) and percent distribution) for T-H cells and higher values for cytotoxic T cells, with reductions in populations of naive T-H and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated T-H and cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of T-H and cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated T-H and cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male. Conclusion: Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for T-H cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated T-H, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies. (J Allergy Clin Immunol 2010;126:1294-301.) 050 UR174 Cambodge / Thaïlande