@article{fdi:010052938,
  title = {{C}ombined eukaryotic and bacterial community fingerprinting of natural freshwater biofilms using automated ribosomal intergenic spacer analysis},
  author = {{F}echner, {L}. {C}. and {V}incent-{H}ubert, {F}. and {G}aubert, {P}hilippe and {B}ouchez, {T}. and {G}ourlay-{F}rance, {C}. and {T}usseau-{V}uillemin, {M}. {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}iofilms are complex communities playing an important role in aquatic ecosystems. {A}utomated ribosomal intergenic spacer analysis ({ARISA}) has been used successfully to explore biofilm bacterial diversity. {H}owever, a gap remains to be filled as regards its application to biofilm eukaryotic populations. {T}he aim of this study is to use {ARISA} to detect eukaryotic population shifts in biofilm. {W}e designed a new set of primers to focus specifically on the {ITS}1-5.8{S}-{ITS}2 region of diatoms and tested it on natural biofilms. {A}dditionally, we tested universal primers, used previously to perform {ARISA} on fungal communities. {C}loning and sequencing showed that the universal primer set amplified various eukaryotes, whereas the new set was diatom specific. {T}he new set amplified a wider variety of diatoms. {T}herefore, the universal set is appropriate to study the general eukaryotic population shifts in biofilms, whereas the new set is more appropriate to study diatoms specifically. {W}e used both primer sets, along with a bacterial set, to study the population shifts in natural river biofilms. {P}rincipal component analysis of the {ARISA} fingerprints revealed seasonal shifts that did not coincide for bacterial and eukaryotic communities. {T}herefore, the use of both eukaryotic and bacterial primers provides a useful insight to assess microbial succession in biofilms.},
  keywords = {{ARISA} ; freshwater periphyton ; eukaryotic community ; bacterial community},
  booktitle = {},
  journal = {{F}ems {M}icrobiology {E}cology},
  volume = {74},
  numero = {3},
  pages = {542--553},
  ISSN = {0168-6496},
  year = {2010},
  DOI = {10.1111/j.1574-6941.2010.00968.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052938},
}