@article{fdi:010052937,
  title = {{T}he nod factor-independent symbiotic signaling pathway : development of {A}grobacterium rhizogenes-mediated transformation for the legume {A}eschynomene indica},
  author = {{B}onaldi, {K}. and {G}herbi, {H}assen and {F}ranche, {C}laudine and {B}astien, {G}. and {F}ardoux, {J}o{\¨e}l and {B}arker, {D}. and {G}iraud, {E}ric and {C}artieaux, {F}abienne},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he nitrogen-fixing symbiosis between {A}eschynomene indica and photosynthetic bradyrhizobia is the only legume-rhizobium association described to date that does not require lipocluto-oligosaccharide {N}od factors ({NF}) {T}o assist in deciphering the molecular basis of this {NF} independent interaction, we have developed a protocol for {A}grobacterium rhizogenes-mediated transformation of {A} indica {T}he co transformation frequency (79%), the nodulation efficiency of transgenic roots (90%), and the expression pattern of the 35{S} {C}auliflower mosaic virus promoter in transgenic nodules were all comparable to those obtained for model legumes {W}e have made use of this tool to monitor the heterologous spatio-temporal expression of the p{M}t{ENOD}11-beta-glucuromdase fusion, a widely used molecular reporter for rhizobial infection and nodulation in both legumes and actinorhizal plants {W}hile {M}t{ENOD}11 promoter activation was not observed in {A} indica roots prior to nodulation, strong reporter-gene expression was observed in the invaded cells of young nodules and in the cell layers bordering the central zone of older nodules {W}e conclude that p{M}t{ENOD}11 expression can be used as an infection related marker in {A} indica and that {A}grobacterium rhizogenes mediated root transformation of {A}eschynomene spp will be an {I}nvaluable tool for determining the molecular basis of the {NF}-{I}ndependent symbiosis},
  keywords = {},
  booktitle = {},
  journal = {{M}olecular {P}lant-{M}icrobe {I}nteractions},
  volume = {23},
  numero = {12},
  pages = {1537--1544},
  ISSN = {0894-0282},
  year = {2010},
  DOI = {10.1094/mpmi-06-10-0137},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052937},
}