@article{fdi:010052920,
  title = {{G}enetic and biological analysis of {C}olombian {P}hthorimaea operculella {G}ranulovirus isolated from {T}ecia solanivora ({L}epidoptera : {G}elechiidae)},
  author = {{E}spinel-{C}orreal, {C}. and {L}{\'e}ry, {X}avier and {V}illamizar, {L}. and {G}omez, {J}. and {Z}eddam, {J}ean-{L}ouis and {C}otes, {A}. {M}. and {L}opez-{F}erber, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ecia solanivora ({L}epidoptera: {G}elechiidae) is an invasive potato pest of the north of {S}outh {A}merica that recently colonized zones where {P}hthorimaea operculella ({L}epidoptera: {G}elechiidae), a taxonomically related insect, was established. {N}owadays, both species can be found in most areas in different proportions. {T}he {P}hthorimaea operculella granulovirus ({P}hop{GV}) was found to efficiently control {P}. operculella and was used as a biopesticide in storage conditions. {H}owever, no appropriate biological control methods exist for {T}. solanivora, and the use of granulovirus isolates would provide a solution. {T}he {C}olombian {C}orporation for {A}gricultural {R}esearch ({CORPOICA}) carried out several {T}. solanivora larva samplings in {C}olombia with the aim of finding potential isolates. {F}ive geographical granulovirus isolates from {T}. solanivora ({VG}001, {VG}002, {VG}003, {VG}004, and {VG}005) were found, and molecular analysis by {REN} profiles shows three different genotypic variants in {C}olombia. {A}nalysis of their genomes revealed their relatedness to {P}hop{GV}. {T}wo isolates exhibited submolar bands in their {REN} patterns, suggesting a mixture of viral genotypes. {T}hese data were confirmed by {PCR} amplification and sequencing of particular regions of the viral genomes. {T}heir biological activity was assayed on both hosts, {T}. solanivora and {P}. operculella. {A} significantly higher pathogenicity in both hosts was observed with isolates {VG}001 and {VG}005 than with isolate {VG}003 or a {P}eruvian isolate (from {P}. operculella) used as a reference in the bioassay. {B}ased on their molecular and biological activity characteristics, {VG}001 and {VG}005 isolates should be selected for further analysis in order to establish their potential as biological control agents.},
  keywords = {},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {76},
  numero = {22},
  pages = {7617--7625},
  ISSN = {0099-2240},
  year = {2010},
  DOI = {10.1128/aem.00999-10},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052920},
}