Yakhlef S. E. B. auteur aut IRD Mousain D. auteur aut IRD Duponnois Robin auteur aut IRD Ducousso M. auteur aut IRD Belkouri A. auteur aut IRD Kerdouh B. auteur aut IRD Perrineau M. M. auteur aut IRD Abourouh M. auteur aut IRD text journalArticle eng text/pdf born digital access The phylogenetic relationships among 200 Pisolithus basidiomata collected from pine, oak, and eucalypt forests and rockrose scrubs in Morocco were investigated. Using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA, this collection could be divided into 5 groups, by using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA. The ITS of a representative basidioma of each group was sequenced, and a phylogenetic analysis was performed. The dendrogram suggests that at least five Pisolithus species are present in Morocco. Pisolithus basidiomata collected in the Pinus and Quercus forests correspond to Pisolithus arrhizus and P. species 4 as previously described by Martin and colleagues in 2002. Those collected from the eucalyptus forests, under E. gomphocephala and E. camaldulensis, correspond to P. albus and P. microcarpus. Basidiomata collected from the rockrose scrubs, under Cistus crispus, C. monspeliensis or C. salviifolius, are all identified as Pisolithus species 3. Phylogenetic analyses showed that our different Pisolithus grouped well with Pisolithus specimens from other geographical origins. specialized Pisolithus ectomycorrhizal fungus phylogenetic analysis ITS of ribosomal DNA sequencing taxonomy ectomycorrhizal fungus pisolithus genetic-variability growth-stimulation ribosomal dna tinctorius eucalyptus diversity australia pcr morphology 084 49 3 157-162 2009 0334-5114 https://www.documentation.ird.fr/hor/fdi:010052903 10.1007/s13199-009-0043-9 0334-5114 [F B010052903] https://www.documentation.ird.fr/hor/fdi:010052903 https://www.documentation.ird.fr/intranet/publi/2010/11/010052903.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-12-03 2017-08-23 fdi:010052903 fre