@article{fdi:010052506,
  title = {{E}arly {HIV}-1 diagnosis using in-house real-time {PCR} amplification on dried blood spots for infants in remote and resource-limited settings},
  author = {{N}go-{G}iang-{H}uong, {N}icole and {K}hamduang, {W}oottichai and {L}eurent, {B}aptiste and {C}ollins, {I}ntira and {N}antasen, {I}ssaren and {L}eechanachai, {P}. and {S}irirungsi, {W}. and {L}imtrakul, {A}. and {L}eusaree, {T}. and {C}omeau, {A}. {M}. and {L}allemant, {M}arc and {J}ourdain, {G}onzague},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: in resource-limited settings, most perinatally {HIV}-1-infected infants do not receive timely antiretroviral therapy because early {HIV}-1 diagnosis is not available or affordable. {O}bjective: {T}o assess the performance of a low-cost in-house realtime polymerase chain reaction ({PCR}) assay to detect {HIV}-1 {DNA} in infant dried blood spots ({DBS}). {M}ethods: {O}ne thousand three hundred nineteen {DBS} collected throughout {T}hailand from non-breast-fed infants born to {HIV}-1-infected mothers were shipped at room temperature to a central laboratory. {I}n-house real-time {DNA} {PCR} results were compared with {R}oche {A}mplicor {HIV}-1 {DNA} test ({V}ersion 1.5) results. {I}n addition, we verified the {R}oche test performance on {DBS} sampled from 1218 other infants using as reference {HIV} serology result at 18 months of age. {R}esults: {R}eal-time {DNA} {PCR} and {R}oche {DNA} {PCR} results were 100% concordant. {C}ompared with {HIV} serology results, the {R}oche test sensitivity was 98.6% (95% confidence interval: 92.6% to 100.0%) and its specificity at 4 months of age was 99.7% (95% confidence interval: 99.2% to 99.9%). {C}onclusions: {I}n-house real-time {PCR} performed as well as the {R}oche test in detecting {HIV}-1 {DNA} on {DBS} in {T}hailand. {C}ombined use of {DBS} and real-time {PCR} assays is a reliable and affordable tool to expand access to early {HIV}-1 diagnosis in remote and resource-limited settings, enabling timely treatment for {HIV}-1-infected infants.},
  keywords = {access to treatment ; dried blood spots ; early diagnosis ; {HIV}-1 real-time {DNA} {PCR} ; infant ; remote and resource-limited settings},
  booktitle = {},
  journal = {{J}aids - {J}ournal of {A}cquired {I}mmune {D}eficiency {S}yndromes},
  volume = {49},
  numero = {5},
  pages = {465--471},
  ISSN = {1525-4135},
  year = {2008},
  DOI = {10.1097/{QAI}.0b013e31818e2531},
  URL = {https://www.documentation.ird.fr/hor/fdi:010052506},
}