Arvold N. D. auteur aut IRD Ngo Giang Huong Nicole auteur aut IRD McIntosh K. auteur aut IRD Suraseranivong V. auteur aut IRD Warachit B. auteur aut IRD Piyaworawong S. auteur aut IRD Changchit T. auteur aut IRD Lallemant Marc auteur aut IRD Jourdain Gonzague auteur aut IRD text journalArticle eng text/pdf born digital access While many factors contribute to mother-to-child transmission (MTCT) of HIV-1, maternal plasma HIV-1 RNA viral load (RNA-VL) has been consistently found as the main risk factor, including when antiretroviral prophylaxis was used to prevent MTCT. However the predictive value of RNA-VL is poor. A recent study of HIV-1-positive pregnant women who did not receive antiretroviral prophylaxis reported an association between HIV-1 DNA viral load (DNA-VL) and MTCT that was stronger than the association between RNA-VL and MTCT. We sought to determine if HIV-1 DNA-VL was independently associated with MTCT of HIV in a population of women who received zidovudine prophylaxis during pregnancy and whose infants received zidovudine after birth. Patients were 33 non-breastfeeding transmitting (TR) and 33 nontransmitting mothers (NTR) from Perinatal HIV Prevention Trial (PHPT-1), a multicenter clinical trial conducted in Thailand comparing zidovudine prophylaxis durations to prevent MTCT. TR and NTR mothers were matched according to baseline RNA-VL. Maternal peripheral blood mononuclear cell (PBMC)-associated HIV-1 DNA was extracted from whole blood, and DNA-VL was established by quantitative real-time polymerase chain reaction. We found that TR had a significantly higher cell-associated HIV-1 DNA viral load than did NTR. Median TR DNA-VL was 2.54 log(10) copies per microgram PBMC DNA, while it was 2.28 log(10) copies per microgram PBMC DNA in NTR (Wilcoxon p = 0.02). In summary, HIV-1 DNA viral load was associated with MTCT in a population of women who received antiretroviral prophylaxis during pregnancy, independently from RNA viral load. specialized 052 056 21 9 638-643 2007 1087-2914 https://www.documentation.ird.fr/hor/fdi:010052476 10.1089/apc.2006.0169 1087-2914 [F B010052476] https://www.documentation.ird.fr/hor/fdi:010052476 https://www.documentation.ird.fr/intranet/publi/depot/2011-09-08/010052476.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-12-03 2017-08-23 fdi:010052476 fre