@article{fdi:010051883,
  title = {{M}echanisms involved in the swelling of erythrocytes caused by {P}acific and {C}aribbean ciguatoxins},
  author = {{S}auviat, {M}.{P}. and {B}oydron {L}e {G}arrec, {R}apha{\¨e}lle and {M}asson, {J}.{B}. and {L}ewis, {R}.{L}. and {V}ernoux, {J}.{P}. and {M}olgo, {J}. and {L}aurent, {D}ominique and {B}enoit, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he mechanisms underlying the swelling of frog red blood cells ({RBC}), induced by {P}acific ({P}-{CTX}-1) and {C}aribbean ({C}-{CTX}-1) ciguatoxins ({CTX}s), were investigated by measuring the length, width and surface of their elliptic shape. {P}-{CTX}-1 (0.5 to 5 n{M}) and {C}-{CTX}-1 (1 mu {M}) induced {RBC} swelling within 60 min. {T}he {CTX}s-induced {RBC} swelling was blocked by apamin (1 mu {M}) and by {S}r2+ (1 mu {M}). {P}-{CTX}-1-induced {RBC} swelling was prevented and inhibited by {H}-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one(27 mu {M}), an inhibitor {O}f {S}oluble guanylate cyclase (s{GC}), and {NOS} blockade by {NG} methyl-{L}-arginine ({L}-{NMA}; 10 mu {M}). {C}ytochalasin {D} (cyt{D}, 10 mu {M}) increased {RBC} surface and mimicked {CTX} effect but did not prevent the {P}-{CTX}-1-induced {L}-{NMA}-sensitive extra increase. {C}alculations revealed that {P}-{CTX}-1 and cyt{D} increase {RBC} total surface envelop and volume. {T}hese data strongly suggest that the molecular mechanisms underlying {CTX}s-induced {RBC} swelling involve the {NO} pathway by an activation of the inducible {NOS}, leading to s{GC} activation which modulates intracellular c{GMP} and regulates {L}-type {C}a2+ channels. {T}he resulting increase in intracellular {C}a2+ content, in turn, disrupts the actin cytoskeleton, which causes a water influx and triggers a {C}a2+-activated {K}+ current through {SK}2 isoform channels. (c) 2005 {E}lsevier {I}nc. {A}ll rights reserved.},
  keywords = {ciguatoxins ; red blood cells ; cell swelling ; {L} type {C}a2+ channels ; nitric oxide ; nitric oxide synthase ; soluble guanylate cyclase ; cytochalasin {D}},
  booktitle = {},
  journal = {{B}lood {C}ells {M}olecules and {D}iseases},
  volume = {36},
  numero = {1},
  pages = {1--9},
  ISSN = {1079-9796},
  year = {2006},
  DOI = {10.1016/j.bcmd.2005.10.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010051883},
}