@article{fdi:010050496,
  title = {{N}ew determinants for tolerance of coffee ({C}offea arabica {L}.) seeds to liquid nitrogen exposure},
  author = {{D}ussert, {S}t{\'e}phane and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he present work establishes for the first time that tolerance of coffee seeds to liquid nitrogen ({LN}) exposure depends on the initial quality of the seedlot and on the rewarming regime employed. {S}eedlot quality was estimated by the parameters of a quantal response model of desiccation sensitivity developed previously. {T}he percentage of seedlings recovered from cryopreserved seeds was very well correlated with the relative humidity ({RH}) at which 90% of the initial viability was retained, {RH}90, as estimated by the model. {W}hatever the cooling regime employed, rewarming the seeds slowly by exposing them to ambient air was highly detrimental. {S}low rewarming-induced viability loss was not due to imbibitional damage since seeds pre-heated at 37 degrees {C} after slow rewarming to 0 degrees {C} exhibited a survival percentage lower than seeds thawed rapidly to 0 degrees {C} before sowing. {T}he optimal hydration status for coffee seed cryopreservation was also re-examined. {D}rying seeds in 81%{RH} provided survival percentages considerably higher than those obtained using the drying {RH} always employed until now, i.e. 78%. {A} new procedure for slowly precooling the seeds prior to immersion in {LN} was also established. {I}t consisted of placing the vials containing the seeds in a dry ice-bath for 25 min., {U}sing this procedure in combination with seed drying in 81% {RH} and rapid rewarming in a 37 degrees {C} water-bath for 30 min ensured the highest survival percentages ever obtained with coffee seeds, i.e. 89%, a value which was not significantly different from the initial viability percentage.},
  keywords = {},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {27},
  numero = {3},
  pages = {169--178},
  ISSN = {0143-2044},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010050496},
}