@article{fdi:010049688,
  title = {{E}ffect of the endogeic earthworm {P}ontoscolex corethrurus on the microbial structure and activity related to {CO}2 and {N}2{O} fluxes from a tropical soil ({M}adagascar)},
  author = {{C}hapuis {L}ardy, {L}ydie and {B}rauman, {A}lain and {B}ernard, {L}aetitia and {P}ablo, {A}nne-{L}aure and {T}oucet, {J}o{\¨e}le and {M}ano, {M}. {J}. and {W}eber, {L}. and {B}runet, {D}idier and {R}azafimbelo, {T}. and {C}hotte, {J}ean-{L}uc and {B}lanchart, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he objective of this laboratory study was to determine the influence of a tropical endogeic earthworm, {P}ontoscolex corethrurus, on {CO}2 and {N}2{O} fluxes from a tropical {F}erralsol and microorganisms potentially involved in these gases emissions. {CO}2 and {N}2{O} fluxes were measured during 35 days from soil mesocosms with and without earthworms. {A}t the end of the incubation, 7% of soil was egested as cast in the earthworm treatment. {T}hen, casts which may be aged from few hours to 35 clays old were isolated from non-ingested soil. {D}ifferent descriptive parameters (activity, density, and structure) of the microbial communities were investigated in the control, the non-ingested soils, and the casts. {Q}uantitative {PCR} of denitrification genes encoding the nitrite (nir{K}) anti nitrous oxide (nos{Z}) reductases was used to study denitrifier density in the earthworm casts. {T}he presence of {P}. corethrurus induced a significant increase in {CO}2 emissions but did not affect {N}2{O} fluxes when measured at mesocosm level. {D}espite the absence of significant differences in {C} and {N} contents between soils and casts, the near infra-red spectra analysis clearly underlined a specific organic signature for the casts. {F}ungal and bacterial biomass significantly decreased (similar to 2-fold) in casts compared to parent soil, but the fungal-to-bacterial ratio was not modified by the earthworm casting activity. {D}ata suggested that bacterial communities, especially denitrifiers, were modified in casts. {T}he relative abundance of nir{K} and nos{Z} genes increased in the casts while the genetic structures of total bacteria and denitrifying communities were slightly modified in the casts. {T}his study highlighted the importance of earthworm casts as a specific soil habitat where a subset of soil functional bacterial communities (such as denitrifiers) found favourable condition for their growth. {H}owever the effect of {P}. corethrurus was less evident when up-scaling from casts to mesocosm level.},
  keywords = {{D}enitrification genes ; {FAME} ; {M}adagascar ; {M}icrobial activity potentials ; {P}ontoscolex corethrurus ; {Q}uantitative {PCR}},
  booktitle = {},
  journal = {{A}pplied {S}oil {E}cology},
  volume = {45},
  numero = {3},
  pages = {201--208},
  ISSN = {0929-1393},
  year = {2010},
  DOI = {10.1016/j.apsoil.2010.04.006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049688},
}