@article{fdi:010049656,
  title = {{I}n planta gene expression analysis of {X}anthomonas oryzae pathovar oryzae, {A}frican strain {MAI}1},
  author = {{S}oto-{S}uarez, {M}auricio and {B}ernal, {D}. and {G}onzalez, {C}arolina and {S}zurek, {B}oris and {G}uyot, {R}omain and {T}ohme, {J}. and {V}erdier, {V}al{\'e}rie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {B}acterial leaf blight causes significant yield losses in rice crops throughout {A}sia and {A}frica. {A}lthough both the {A}sian and {A}frican strains of the pathogen, {X}anthomonas oryzae pv. oryzae ({X}oo), induce similar symptoms, they are nevertheless genetically different, with the {A}frican strains being more closely related to the {A}sian {X}. oryzae pv. oryzicola ({X}oc). {R}esults: {C}hanges in gene expression of the {A}frican {X}oo strain {MAI}1 in the susceptible rice cultivar {N}ipponbare were profiled, using an {SSH} {X}oo {DNA} microarray. {M}icroarray hybridization was performed comparing bacteria recovered from plant tissues at 1, 3, and 6 days after inoculation (dai) with bacteria grown in vitro. {A} total of 710 bacterial genes were found to be differentially expressed, with 407 up-regulated and 303 down-regulated. {E}xpression profiling indicated that less than 20% of the 710 bacterial transcripts were induced in the first 24 h after inoculation, whereas 63% were differentially expressed at 6 dai. {T}he 710 differentially expressed genes were one-end sequenced. 535 sequences were obtained from which 147 non-redundant sequences were identified. {D}ifferentially expressed genes were related to metabolism, secretion and transport, pathogen adherence to plant tissues, plant cell-wall degradation, {IS} elements, and virulence. {I}n addition, various other genes encoding proteins with unknown function or showing no similarity to other proteins were also induced. {T}he {X}oo {MAI}1 non-redundant set of sequences was compared against several {X}. oryzae genomes, revealing a specific group of genes that was present only in {MAI}1. {N}umerous {IS} elements were also found to be differentially expressed. {Q}uantitative real-time {PCR} confirmed 86% of the identified profile on a set of 14 genes selected according to the microarray analysis. {C}onclusions: {T}his is the first report to compare the expression of {X}oo genes in planta across different time points during infection. {T}his work shows that as-yet-unidentified and potentially new virulence factors are appearing in an emerging {A}frican pathogen. {I}t also confirms that {A}frican {X}oo strains do differ from their {A}sian counterparts, even at the transcriptional level.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{B}mc {M}icrobiology},
  volume = {10},
  numero = {},
  pages = {170},
  ISSN = {1471-2180},
  year = {2010},
  DOI = {10.1186/1471-2180-10-170},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049656},
}