@article{fdi:010049618,
  title = {{P}lasmodium falciparum population dynamics in a cohort of pregnant women in {S}enegal},
  author = {{G}uitard, {J}uliette and {A}ndersen, {P}. and {E}rmont, {C}aroline and {G}nidehou, {S}{\'e}dami and {F}ievet, {N}adine and {L}und, {O}. and {D}eloron, {P}hilippe and {T}uikue {N}dam, {N}icaise},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}regnant women acquire protective antibodies that cross-react with geographically diverse placental {P}lasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria ({PAM}) parasites have conserved epitopes and, that designing a {PAM} vaccine may be envisaged. {VAR}2{CSA} is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. {M}ethods: {T}he dynamics of {P}. falciparum genotypes during pregnancy in 32 women in relation to {VAR}2{CSA} polymorphism and immunity was determined. {T}he polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the {DBL}5 epsilon + {I}nterdomain 5 ({I}d5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. {R}esults: {I}n primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. {S}ome parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-{VAR}2{CSA} antibody level was low. {C}omparison of diversity among genotyping markers confirmed that some {PAM} parasites may harbour more than one var2csa gene copy in their genome. {C}onclusions: {H}ost immunity to {VAR}2{CSA} influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. {P}resence of highly conserved residues in surface-exposed areas of the {VAR}2{CSA} immunodominant {DBL}5 epsilon domain, suggest its potential in inducing antibodies with broad reactivity.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {9},
  numero = {},
  pages = {165},
  ISSN = {1475-2875},
  year = {2010},
  DOI = {10.1186/1475-2875-9-165},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049618},
}