@article{fdi:010049558,
  title = {{G}enomic comparison between {X}anthomonas oryzae pv. oryzae and {X}anthomonas oryzae pv. oryzicola, using suppression-subtractive hybridization},
  author = {{S}oto-{S}uarez, {M}auricio and {G}onzalez, {C}. and {P}i{\'e}gu, {B}. and {T}ohme, {J}. and {V}erdier, {V}al{\'e}rie},
  editor = {},
  language = {{ENG}},
  abstract = {{X}anthomonas oryzae pathovar oryzae ({X}oo) and {X}. oryzae pv. oryzicola ({X}oc) cause bacterial diseases in rice: leaf blight and leaf streak, respectively. {A}lthough both the {A}sian and the {A}frican strains of {X}oo induce similar symptoms, they are genetically different, with the {A}frican {X}oo strains being more closely related to the {A}sian {X}oc. {T}o identify the sequences responsible for differences between {A}frican and {A}sian {X}oo strains and their relatedness to {X}oc strains, a suppression-subtractive hybridization ({SSH}) procedure was performed, using the {A}frican {X}oo {MAI}1 strain as a tester and the {P}hilippine {X}oo {PXO}86 strain and {X}oc {BLS}256 strain as drivers. {A} nonredundant set of 134 sequences from {MAI}1 was generated. {S}everal {DNA} fragments isolated by {SSH} were similar to genes of unknown function, hypothetical proteins, genes related to the type {III} secretion system, and other pathogenicity-related genes. {T}he specificity of various fragments was validated by {S}outhern blot analysis. {SSH} sequences were compared with several xanthomonad genomes. {I}n silico analysis revealed {SSH} sequences as specific to strain {MAI}1, revealing their potential as specific markers for further epidemiological and diagnostic studies. {SSH} proved to be a useful method for rapidly identifying specific genes among closely related {X}. oryzae strains.},
  keywords = {{X}anthomonas oryzae pv oryzae ; {X}anthomonas oryzae pv oryzicola ; subtractive library ; specific genes ; virulence genes},
  booktitle = {},
  journal = {{F}ems {M}icrobiology {L}etters},
  volume = {308},
  numero = {1},
  pages = {16--23},
  ISSN = {0378-1097},
  year = {2010},
  DOI = {10.1111/j.1574-6968.2010.01985.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049558},
}