@article{fdi:010049517,
  title = {{I}nfestation of peridomestic {A}ttalea phalerata palms by {R}hodnius stali, a vector of {T}rypanosoma cruzi in the {A}lto {B}eni, {B}olivia},
  author = {{J}usti, {S}. {A}. and {N}oireau, {F}ran{\c{c}}ois and {C}ortez, {M}. {R}. and {M}onteiro, {F}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{OBJECTIVES} {T}o determine (i) whether peridomestic {A}ttalea phalerata palms in fragmented human-occupied areas of the {A}lto {B}eni, {B}olivia, are infested by triatomines; (ii) the specific status of triatomines captured in the area; and (iii) the rate of natural {T}rypanosoma cruzi infection among those triatomines. {METHODS} {O}ne hundred and twenty-five live-bait traps were used to sample 47 {A}. phalerata palms in three {A}lto {B}eni localities. {A}ctive search for vectors was also performed in 10 chicken coops and three rice storage units. {O}nly {R}hodnius specimens were found. {A}s nymphs of closely related {R}hodnius species are morphologically undistinguishable, and because of controversy in the literature regarding which {R}hodnius species occur in {B}olivia, collected insects were identified through molecular taxonomy. {P}hylogenetic analyses of {DNA} sequences obtained for a fragment of the mitochondrial cytochrome b gene and for the nuclear {ITS}-2 ribosomal region were used as molecular markers. {N}atural infection rates were determined using a pair of primers that {PCR}-amplify a 330-bp fragment of the parasite's k{DNA}. {RESULTS} {T}welve nymphs were captured in five {A}. phalerata palms (from two of the three localities studied), and an adult was collected from a chicken coop in {I}niqua (and morphologically identified as {R}hodnius stali). {A}ll nymphs (as well as the adult) were molecularly identified as {R}. stali based on the two molecular markers used. {A} single nymph was found to be infected with {T}. cruzi. {CONCLUSIONS} {A}ttalea phalerata palms represent an important sylvatic ecotope occupied by {R}. stali in the {A}lto {B}eni region of {B}olivia, where there are signs of {T}. cruzi transmission to humans, despite the preliminary indication of low level of natural infection of the vectors.},
  keywords = {{R}hodnius stali ; {C}hagas disease ; {B}olivia ; cytochrome b ; {ITS}-2 ; molecular ; taxonomy},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {15},
  numero = {6},
  pages = {727--732},
  ISSN = {1360-2276},
  year = {2010},
  DOI = {10.1111/j.1365-3156.2010.02527.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049517},
}