@article{fdi:010049494,
  title = {{I}nsecticide resistance status in {A}nopheles gambiae in southern {B}enin},
  author = {{Y}adouleton, {A}. {W}. and {P}adonou, {G}. and {A}sidi, {A}. and {M}oiroux, {N}icolas and {B}io-{B}anganna, {S}. and {C}orbel, {V}incent and {N}'{G}uessan, {R}. and {G}benou, {D}. and {Y}acoubou, {I}. and {G}azard, {K}. and {A}kogbeto, {M}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he emergence of pyrethroid resistance in {A}nopheles gambiae has become a serious concern to the future success of malaria control. {I}n {B}enin, the {N}ational {M}alaria {C}ontrol {P}rogramme has recently planned to scaling up long-lasting insecticidal nets ({LLIN}s) and indoor residual spraying ({IRS}) for malaria prevention. {I}t is, therefore, crucial to monitor the level and type of insecticide resistance in {A}n. gambiae, particularly in southern {B}enin where reduced efficacy of insecticide-treated nets ({ITN}s) and {IRS} has previously been reported. {M}ethods: {T}he protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern {B}enin. {B}ioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, {DDT} 4%, and bendiocarb 0.1%) following {WHOPES} guidelines. {T}he species within {A}n. gambiae complex, molecular form and presence of kdr and ace-1 mutations were determined by {PCR}. {R}esults: {S}trong resistance to permethrin and {DDT} was found in {A}n. gambiae populations from southern {B}enin, except in {A}glangandan where mosquitoes were fully susceptible (mortality 100%) to all insecticides tested. {PCR} showed the presence of two sub-species of {A}n. gambiae, namely {A}n. gambiae s.s, and {A}nopheles melas, with a predominance for {A}n. gambiae s.s (98%). {T}he molecular {M} form of {A}n. gambiae was predominant in southern {B}enin (97%). {T}he kdr mutation was detected in all districts at various frequency (1% to 95%) whereas the {A}ce-1 mutation was found at a very low frequency (<= 5%). {C}onclusion: {T}his study showed a widespread resistance to permethrin in {A}n. gambiae populations from southern {B}enin, with a significant increase of kdr frequency compared to what was observed previously in {B}enin. {T}he low frequency of {A}ce-1 recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to pyrethroids for {IRS} in {B}enin.},
  keywords = {{BENIN}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {9},
  numero = {},
  pages = {83 [6 ]},
  ISSN = {1475-2875},
  year = {2010},
  DOI = {10.1186/1475-2875-9-83},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049494},
}