Corrales Rosa Milagros auteur aut IRD Mathieu-Daudé Françoise auteur aut IRD Garcia Déborah auteur aut IRD Brenière Simone Frédérique auteur aut IRD Sereno Denis auteur aut IRD text journalArticle eng text/pdf born digital access Extracellular factors produced by Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei are important in the host-parasite relationship. Here, we describe a genome-based approach to identify putative extracellular proteins conserved among trypanosomatids that are likely involved in the classical secretory pathway. Potentially secreted proteins were identified by bioinformatic analysis of the T. cruzi genome. A subset of thirteen genes encoding unknown proteins with orthologs containing a signal peptide sequence in L. infantum, L. major, and T. brucei were transfected into L. infantum. Tagged proteins detected in the extracellular medium confirmed computer predictions in about 25% of the hits. Secretion was confirmed for two L. infantum orthologs proteins using the same experimental system. Infectivity studies of transgenic Leishmania parasites suggest that one of the secreted proteins increases parasite replication inside macrophages. This methodology can identify conserved secreted proteins involved in the classical secretory pathway, and they may represent potential virulence factors in trypanosomatids. specialized 052 752698 2010 1110-7243 https://www.documentation.ird.fr/hor/fdi:010049373 10.1155/2010/752698 1110-7243 [F B010049373] https://www.documentation.ird.fr/hor/fdi:010049373 https://www.documentation.ird.fr/intranet/publi/2010/03/010049373.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-04-06 2017-08-23 fdi:010049373 fre