@article{fdi:010049373,
  title = {{A}n experimental approach for the identification of conserved secreted proteins in trypanosomatids},
  author = {{C}orrales, {R}osa {M}ilagros and {M}athieu-{D}aud{\'e}, {F}ran{\c{c}}oise and {G}arcia, {D}{\'e}borah and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique and {S}ereno, {D}enis},
  editor = {},
  language = {{ENG}},
  abstract = {{E}xtracellular factors produced by {L}eishmania spp., {T}rypanosoma cruzi, and {T}rypanosoma brucei are important in the host-parasite relationship. {H}ere, we describe a genome-based approach to identify putative extracellular proteins conserved among trypanosomatids that are likely involved in the classical secretory pathway. {P}otentially secreted proteins were identified by bioinformatic analysis of the {T}. cruzi genome. {A} subset of thirteen genes encoding unknown proteins with orthologs containing a signal peptide sequence in {L}. infantum, {L}. major, and {T}. brucei were transfected into {L}. infantum. {T}agged proteins detected in the extracellular medium confirmed computer predictions in about 25% of the hits. {S}ecretion was confirmed for two {L}. infantum orthologs proteins using the same experimental system. {I}nfectivity studies of transgenic {L}eishmania parasites suggest that one of the secreted proteins increases parasite replication inside macrophages. {T}his methodology can identify conserved secreted proteins involved in the classical secretory pathway, and they may represent potential virulence factors in trypanosomatids.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {B}iomedicine and {B}iotechnology},
  numero = {},
  pages = {752698},
  ISSN = {1110-7243},
  year = {2010},
  DOI = {10.1155/2010/752698},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049373},
}