@article{fdi:010049356,
  title = {{F}low cytometry for the evaluation of anti-plasmodial activity of drugs on {P}lasmodium falciparum gametocytes},
  author = {{C}hevalley, {S}{\'e}verine and {C}oste, {A}. and {L}opez, {A}. and {P}ipy, {B}. and {V}alentin, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he activity of promising anti-malarial drugs against {P}lasmodium gametocytes is hard to evaluate even in vitro. {T}his is because visual examination of stained smears, which is commonly used, is not totally convenient. {I}n the current study, flow cytometry has been used to study the effect of established anti-malarial drugs against sexual stages obtained from {W}2 strain of {P}lasmodium falciparum. {G}ametocytes were treated for 48 h with different drug concentrations and the gametocytaemia was then determined by flow cytometry and compared with visual estimation by microscopy. {R}esults and conclusions: {I}nitially gametocytaemia was evaluated either using light microscopy or flow cytometry. {A} direct correlation (r(2) = 0.9986) was obtained. {T}wo distinct peaks were observed on cytometry histograms and were attributed to gametocyte populations. {T}he activities of established anti-malarial compounds were then measured by flow cytometry and the results were equivalent to those obtained using light microscopy. {P}rimaquine and artemisinin had {IC}50 of 17.6 mu {M} and 1.0 mu {M}, respectively. {G}ametocyte sex was apparently distinguishable by flow cytometry as evaluated after induction of exflagellation by xanthurenic acid. {T}hese data form the basis of further studies for developing new methods in drug discovery to decrease malaria transmission.},
  keywords = {},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {9},
  numero = {},
  pages = {49},
  ISSN = {1475-2875},
  year = {2010},
  DOI = {10.1186/1475-2875-9-49},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049356},
}