@article{fdi:010049354,
  title = {{E}xocytosis and protein secretion in {T}rypanosoma},
  author = {{G}eiger, {A}nne and {H}irtz, {C}. and {B}ecue, {T}. and {B}ellard, {E}. and {C}enteno, {D}. and {G}argani, {D}. and {R}ossignol, {M}. and {C}uny, {G}{\'e}rard and {P}eltier, {J}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {H}uman {A}frican trypanosomiasis is a lethal disease caused by the extracellular parasite {T}rypanosoma brucei. {T}he proteins secreted by {T}. brucei inhibit the maturation of dendritic cells and their ability to induce lymphocytic allogenic responses. {T}o better understand the pathogenic process, we combined different approaches to characterize these secreted proteins. {R}esults: {O}verall, 444 proteins were identified using mass spectrometry, the largest parasite secretome described to date. {F}unctional analysis of these proteins revealed a strong bias toward folding and degradation processes and to a lesser extent toward nucleotide metabolism. {T}hese features were shared by different strains of {T}. brucei, but distinguished the secretome from published {T}. brucei whole proteome or glycosome. {I}n addition, several proteins had not been previously described in {T}rypanosoma and some constitute novel potential therapeutic targets or diagnostic markers. {I}nterestingly, a high proportion of these secreted proteins are known to have alternative roles once secreted. {F}urthermore, bioinformatic analysis showed that a significant proportion of proteins in the secretome lack transit peptide and are probably not secreted through the classical sorting pathway. {M}embrane vesicles from secretion buffer and infested rat serum were purified on sucrose gradient and electron microscopy pictures have shown 50- to 100-nm vesicles budding from the coated plasma membrane. {M}ass spectrometry confirmed the presence of {T}rypanosoma proteins in these microvesicles, showing that an active exocytosis might occur beyond the flagellar pocket. {C}onclusions: {T}his study brings out several unexpected features of the secreted proteins and opens novel perspectives concerning the survival strategy of {T}rypanosoma as well as possible ways to control the disease. {I}n addition, concordant lines of evidence support the original hypothesis of the involvement of microvesicle-like bodies in the survival strategy allowing {T}rypanosoma to exchange proteins at least between parasites and/or to manipulate the host immune system.},
  keywords = {{AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{B}mc {M}icrobiology},
  volume = {10},
  numero = {},
  pages = {20},
  ISSN = {1471-2180},
  year = {2010},
  DOI = {10.1186/1471-2180-10-20},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049354},
}