Zeddam Jean-Louis auteur aut IRD Gordon K. H. J. auteur aut IRD Lauber C. auteur aut IRD Alves C. A. F. auteur aut IRD Luke B. T. auteur aut IRD Hanzlik T. N. auteur aut IRD Ward V. K. auteur aut IRD Gorbalenya A. E. auteur aut IRD text journalArticle eng text/pdf born digital access The Tetraviridae is a family of non-enveloped positive-stranded RNA insect viruses that is defined by the T=4 symmetry of virions. We report the complete Euprostema elaeasa virus (EeV) genome sequence of 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding the replicase and capsid precursor, with similar to 67% amino acid identity to Thosea asigna virus (TaV). The N-terminally positioned 17 kDa protein is released from the capsid precursor by a NPGP motif EeV has 40 nm non-enveloped isometric particles composed of 58 and 7 kDa proteins. The 3'-end of TaV/EeV is predicted to form a conserved pseudoknot. Replicases of TaV and EeV include a newly delineated VPg signal mediating the protein priming of RNA synthesis in dsRNA Birnaviridae. Results of rooted phylogenetic analysis of replicase and capsid proteins are presented to implicate recombination between monopartite tetraviruses, involving autonomization of a sgRNA, in the emergence of bipartite tetraviruses. They are also used to revise the Tetraviridae taxonomy. specialized Tetraviridae Birnaviridae Insect virus Genome sequence Permuted RNA-dependent RNA polymerase VPg Jelly-roll-based capsid Phylogeny Recombination Taxonomy 052 397 1 145-154 2010 0042-6822 https://www.documentation.ird.fr/hor/fdi:010049280 10.1016/j.virol.2009.10.042 0042-6822 [F B010049280] https://www.documentation.ird.fr/hor/fdi:010049280 https://www.documentation.ird.fr/intranet/publi/2010/02/010049280.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-03-10 2017-08-23 fdi:010049280 fre