@article{fdi:010049246, title = {{D}rying-rewetting cycles and gamma-irradiation effects on enzyme activities of distinct layers from a {Q}uercus ilex {L}. litter}, author = {{A}larcon-{G}utierrez, {E}. and {F}loch, {C}. and {Z}iarelli, {F}. and {A}ugur, {C}hristopher and {C}riquet, {S}.}, editor = {}, language = {{ENG}}, abstract = {{I}n a {M}editerranean climate, water stress is one of the principal constraints on proper forest ecosystem functioning. {D}rought influences rates of organic matter degradation by affecting microbial growth and enzyme activities. {T}he objectives of this study were: (i) to evaluate the effect of repeated drying-rewetting cycles on cellulase, alkaline phosphatase and fluorescein diacetate ({FDA}) hydrolase activities of three distinct {Q}uercus ilex {L}. litter layers, and (ii) to investigate the effect of these cycles on gamma-irradiated litters in order to distinguish the abiotic influence on the fluctuations observed. {R}esults, for all three layers, showed high correlations between litter water content and enzyme activities. {U}nder mesocosm conditions, and using non-sterilized litter samples, cellulase, alkaline phosphatase, and {FDA} activities significantly decreased or increased during drying or rewetting cycles respectively. {S}ignificant differences were also found when evaluating the effect of litter depth on enzyme activities, the intermediate depth ({OL}v layer) generally being the most active. {F}or gamma-sterilized samples, {FDA} activity still fluctuated with drying-rewetting cycles. {A}ssays showed that pre-humidification of gamma-irradiated litter increased {FDA} activity two-fold in the first 30 min. {A}ll these results have shown that following drying-rewetting cycles, some of the fluctuations occur independently of microbial growth, suggesting abiotic interactions, such as desorption, in combination with both solvatation status and conformational changes of enzymes.}, keywords = {{A}biotic fluctuations ; {D}rying-rewetting ; {E}nzyme activities ; gamma-{I}rradiation ; {M}oisture}, booktitle = {}, journal = {{S}oil {B}iology and {B}iochemistry}, volume = {42}, numero = {2}, pages = {283--290}, ISSN = {0038-0717}, year = {2010}, DOI = {10.1016/j.soilbio.2009.11.005}, URL = {https://www.documentation.ird.fr/hor/fdi:010049246}, }