@article{fdi:010049243,
  title = {{M}orpho-histological study of banana ({M}usa spp. cv. {G}rande {N}aine [{AAA}]) cell suspensions during cryopreservation and regeneration},
  author = {{G}eorget, {F}. and {E}ngelmann, {F}lorent and {D}omergue, {R}. and {C}ote, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this work, a morpho-histological study of banana ({M}usa spp. cv. {G}rande {N}aine [{AAA}]) embryogenic cell suspensions during cryopreservation and regeneration was performed. {I}t was demonstrated that the regeneration process of somatic embryos originating from cryopreserved cell suspensions was different from that of control cell suspensions. {S}omatic embryos originating from cryopreserved cell suspensions had a unicellular origin. {T}he regeneration process was modified not only by freezing in liquid nitrogen but also by the plasmolyzing effect of the 0.5 {M} sucrose solution employed during pretreatment. {T}his result explained the high number of embryonic structures formed on {M}3 medium, compared with the control. {P}roembryos blocked at the globular stage could pursue their development when they were plated on new culture medium at a lower density after 30 days of culture on {M}3 medium. {T}he unicellular origin of somatic embryos produced from cryopreserved cell suspensions offers the prospect of using cryopreservation to select non-chimeral transformed plants.},
  keywords = {banana ; embryogenic cell suspension ; cryopreservation ; histology ; cellular selection},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {30},
  numero = {6},
  pages = {398--407},
  ISSN = {0143-2044},
  year = {2009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049243},
}