@article{fdi:010049207,
  title = {{V}ariations of plasmid content in {R}ickettsia felis},
  author = {{F}ournier, {P}. {E}. and {B}elghazi, {L}. and {R}obert, {C}. and {E}lkarkouri, {K}. and {R}ichards, {A}. {L}. and {G}reub, {G}. and {C}ollyn, {F}. and {O}gawa, {M}. and {P}ortillo, {A}. and {O}teo, {J}. {A}. and {P}saroulaki, {A}. and {B}itam, {I}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {S}ince its first detection, characterization of {R}. felis has been a matter of debate, mostly due to the contamination of an initial {R}. felis culture by {R}. typhi. {H}owever, the first stable culture of {R}. felis allowed its precise phenotypic and genotypic characterization, and demonstrated that this species belonged to the spotted fever group rickettsiae. {L}ater, its genome sequence revealed the presence of two forms of the same plasmid, physically confirmed by biological data. {I}n a recent article, {G}illespie et al. ( {PL}o{S} {O}ne. 2007; 2( 3): e266.) used a bioinformatic approach to refute the presence of the second plasmid form, and proposed the creation of a specific phylogenetic group for {R}. felis. {M}ethodology/ {P}rincipal {F}indings: {I}n the present report, we, and five independent international laboratories confirmed unambiguously by {PCR} the presence of two plasmid forms in {R}. felis strain {URRWXC}al(2)({T}), but observed that the plasmid content of this species, from none to 2 plasmid forms, may depend on the culture passage history of the studied strain. {W}e also demonstrated that {R}. felis does not cultivate in {V}ero cells at 37 degrees {C} but generates plaques at 30 degrees {C}. {F}inally, using a phylogenetic study based on 667 concatenated core genes, we demonstrated the position of {R}. felis within the spotted fever group. {S}ignificance: {W}e demonstrated that {R}. felis, which unambiguously belongs to the spotted fever group rickettsiae, may contain up to two plasmid forms but this plasmid content is unstable.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {3},
  numero = {5},
  pages = {e2289},
  ISSN = {1932-6203},
  year = {2008},
  DOI = {10.1371/journal.pone.0002289},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049207},
}