@article{fdi:010049085,
  title = {{I}dentification of coffee {WRKY} transcription factor genes and expression profiling in resistance responses to pathogens},
  author = {{R}amiro, {D}aniel and {J}alloul, {A}. and {P}etitot, {A}nne-{S}ophie and {G}rossi de {S}a, {M}. {F}. and {M}aluf, {M}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n plants, {WRKY} proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. {T}o assess the diversity of this protein family in coffee ({C}offea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. {A} total of 53 different putative {WRKY} genes were obtained, but only 22 unigenes encoding a protein with a {WRKY} domain were identified, eight of which are supported by full-length c{DNA} sequences. {A}lignment of {WRKY} domain sequences of the coffee unigenes and 72 {A}rabidopsis thaliana {WRKY} genes showed that the 22 coffee {WRKY} members were distributed among the main {A}. thaliana {WRKY} subgroups and shared conserved peptide domains. {T}o assess the involvement of {WRKY} genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. {F}ive members of {WRKY} groups {II}d and {III} were regulated only by pathogens and hormone treatments. {A}lthough a significant correlation of {WRKY} genes expression after {M}e{JA} and rust treatments was observed, expression of coffee genes involved in {JA} biosynthesis and lipoxygenase ({EC} 1.13.11.12) activity assays did not support the involvement of {JA} in the early coffee resistance responses to the rust pathogen. {T}he five {WRKY} transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses.},
  keywords = {{R}ust disease ; {C}offea arabica ; {R}egulator genes},
  booktitle = {},
  journal = {{T}ree {G}enetics and {G}enomes},
  volume = {6},
  numero = {5},
  pages = {767--781},
  ISSN = {1614-2942},
  year = {2010},
  DOI = {10.1007/s11295-010-0290-1},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049085},
}